Research Article

Isolation and Identification of Helicobacter Pylori from Poultry Meat in Lahore, Pakistan

Zainab Masood, Tanveer Majeed

Abstract :

Helicobacter pylori is a gram-negative bacterium that is recognized as a causative agent of major diseases in the gastrointestinal tract, peptic ulcer disease, and gastric cancer. The poultry meat origin has also been a cause of concern due to its zoonotic capability. This study sought to investigate the presence of H. pylori in poultry meat tissue samples obtained from Lahore, Pakistan, and further examined the resistance of isolated bacteria to antibiotics. A total of 50 samples were collected from the home-affairs including casual yet expandable retailing facilities and coded samples were cultured on Brucella blood agar under microaerophilic conditions. Biochemical tests including catalase, urease and indole tests helped to detect only 1 sample of H. pylori confirming its occurrence and lower prevalence level. The isolate was found to be susceptible to ampicillin, kanamycin, but not streptomycin. 34% of the samples exhibited poly – microbial growth; this might have made it difficult to isolate H. pylori in other instances. This suggests that the direct zoonotic impact of poultry meat is low although the potential transmission will persist particularly in areas with poor hygiene. Further research should be considered the geographical expansion of the relationship between H. pylori prevalence and the level of antibiotic resistance for better evaluation of poultry meat public health risk.

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