Research Article
DNA Damage and Chromosomal Aberrations Assessment in Trace Mineral Deficient Patients
Komal Ashraf
Published on : June 2024 | Volume : 1 Issue : 1
Abstract
Minerals play vital roles in human health. They are important in genome stability. Deficiency of macro and micro minerals can lead to high oxidative stress which affects the genomic DNA. This study was aimed to determine the genotoxicity status in mineral deficient patients. A total of 200 blood samples were taken from the confirmed mineral deficient individuals and free of any kind of other diseases. Negative control group included healthy individuals that were not having any kind of deficiency and disease with no smoking habits and drug addiction. Individuals with any kind of disease, smoking habits, or drug addiction were excluded from the study. To evaluate genotoxic effects in mineral-deficient group, Comet assay, micronucleus and chromosomal aberration assay were performed. Among deficient minerals, sodium was found to be highest deficient mineral while phosphorus was found least deficient. Comparison of genotoxicity revealed a significant difference among all performed tests. DNA damage was observed showed highest in the mineral deficient individuals. Negative control individuals were having no significant genotoxic effects. Results of chromosomal aberrations appeared in three distinctive shapes i.e. V-shaped, Breakage or Circular. It was concluded that mineral deficient were having genotoxic effects which can lead to the cancer.